Figure 3.

TCR repertoire in baseline tumor tissues associated with tumor microenvironment.

(a) Patients with high baseline PD-1⁺CD4⁺ TILs had higher Shannon index, lower clonality and lower Simpson’s index in baseline tumors. (b) Patients with high baseline PD-1⁺CD8⁺ TILs had higher Shannon index and lower Simpson’s index in baseline tumors. The median density (/mm²) of PD-1⁺CD4⁺ TILs and PD-1⁺CD8⁺ TILs was used as cutoffs in (a) and (b). (c) Association between TCR diversity and tumor mutation burden. (d) Patients with RTK-RAS pathway mutations had higher Simpson’s index in baseline tumors. (e) Patient-specific and shared unique T-cell clonotypes in individual patient. (f) Unique T-cell clonotypes shared among patients. (g) Landmark analysis of overall survival and progression-free survival among patients in patient-specific unique clonotypes in baseline tumors. TIL, tumor-infiltrated lymphocyte. TMB, tumor mutation burden. N = 17 (a and b), 16 (c and d), and 18 (e-g). P < .05, significant difference.