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. 2022 Jan 13;14(1):2013764. doi: 10.1080/19490976.2021.2013764

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© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — HS administration maintains colonocyte hypoxia by activating epithelial PPAR- γ signaling during colitis. (a-b) Immunofluorescent staining for Hypoxyprobe-1(red) and nuclear (blue) in colon tissue and quantification of staining intensities (n = 8). (Scale bars: 50 μm, Magnification: 40x) (c-e) Western blot was used to determine PPAR- γ and iNOS with β-actin as reference in colon tissue (n = 3). (f) Real time RT-PCR analysis of Nos2 (iNOS) transcript levels from colonic epithelia (n = 3). All data show mean ± SD, are representative of two to four independent experiments, and include statistical significance calculated by one-way ANOVA with LSD multiple comparison post hoc test. ns, no significant; * P < .05; **P < .01; ***P < .001.