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. 2022 Jan 12;219(3):e20210527. doi: 10.1084/jem.20210527

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© 2022 Liu et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 1. — TI GCs collapse without extensive apoptosis. (A) The principle (left) and protocols (right) for constructing TD and TI GCs. (B and C) Abundance of TI and TD GCs. (B) Representative FACS profiles for gating of B1-8^hi TD and TI GCs at indicated time after immunization. (C) Summary statistics of B1-8^hi GC% in B cells. (D and E) Apoptosis in TD and TI GCs. (D) Representative FACS plots of cCasp staining of B1-8^hi GC B cells. (E) Summary statistics of cCasp⁺% in B1-8^hi GCs. Data were pooled from two (B and C) or three (D and E) independent experiments with at least two mice per group. Each symbol indicates one mouse, and lines denote means. P values by Student’s t test. *, P < 0.05; **, P < 0.01. DPI, days postimmunization; FSC, forward scatter.