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. 2022 Jan 14;17(1):e0262500. doi: 10.1371/journal.pone.0262500

Fig 5. Monosaccharide composition in TFA-hydrolyzed supernatants after subsequent treatments in stepwise degradation.

Fig 5

Individual sugar in the supernatant referred to initial biomass (y-axis) after each treatment (x-axis). Di-ammonium oxalate (AmOx): c(AmOx) = 20 mmol L-1, pH = 4, T = 70°C, t = 1 h. 0.1 M NaOH: c(NaOH) = 0.1 mol L-1, T = 22°C, t = 24 h. 4.4 M NaOH: c(NaOH) = 4.4 mol L-1, T = 22°C, t = 8 h. H2SO4: 72% H2SO4, T = 22°C, t = 1 h, followed by dilution with water to 4% H2SO4, T = 100°C, t = 1 h. n = 3. Neutralized supernatants were hydrolyzed by 2 mol L-1 TFA, T = 100°C, t = 1.5 h and analyzed for monosaccharide composition using high-performance anion exchange chromatography.