Fig 2. RIG-I is the main RNA sensor that recognizes m⁶A-deficient RSV virion RNA.

Confluent wild-type (A), RIG-I-knockout (B), MDA5-knockout (C), or MAVS-knockout (D) A549 Dual cells were transfected with same amount (2×10⁷ RNA copies) of RSV virion RNAs from either METTL3 KD cells or METTL3 WT cells. Cell culture supernatants were harvested at 24 and 40 h after inoculation, IFN-β in the supernatant at indicated time points was measured by commercial ELISA kit. Error bars represent SD from n  =  3 independent experiments. *P<0.5, **P < 0.01, ***P < 0.001, ****P < 0.0001.