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. 2021 Dec 20;17(12):e1010142. doi: 10.1371/journal.ppat.1010142

Fig 3. In vitro ubiquitination analysis of RIG-I.

Fig 3

(A) rgRSV-GALL(+/-) virion RNA enhances RIG-I ubiquitination (RIG-I-Ubn) compared to rgRSV virion RNA. 1.0 μM of purified RIG-I was incubated with 1ng/ μl of 42 bp dsRNA or different doses (0.1, 10, and 74.1 ng/ μl) of virion RNA from rgRSV-GALL(+/-) or parental rgRSV with or without RIPLET. Ubiquitination of RIG-I was analyzed by anti-RIG-I blot. (B) Virion RNA from METTL3-KD U2OS cells enhances RIG-I ubiquitination compared to virion RNA from wt U2OS cells. The density of each lane was quantified by Image J. The length of lane used for quantification was indicated by the line in the left side. The ratio between m6A-deficient virion RNA and wild type virion RNA at each RNA concentration is indicated.