Fig 7. Restoration of the replication of m⁶A-deficient rgRSVs in RIG-I and MAVS-knockout A549 cells.

Replication kinetics of m⁶A-deficient rgRSVs in WT (A) RIG-I-knockout (B) or MAVS-knockout (C) A549 cells. Cells in 24-well plates were infected by each rgRSV at an MOI of 1.0, virus was harvested at day 0–3 post-infection, and viral titer was determined by TCID₅₀ assay in HEp-2 cells. Viral titers are the geometric mean titer (GMT) of three independent experiments ± standard deviation. Viral titer of each rgRSV at each time point was compared with that of the parental rgRSV. Statistical significance was determined by two-sided Student’s t-test. *P<0.5, **P < 0.01, ***P < 0.001, ****P < 0.0001.