Fig 9. Viral m⁶A methylation modulates RSV-specific T cell response.

Five-week-old BALB/c mice were immunized with each rgRSV mutant (5 mice per group). Mice were euthanized at 4 weeks post-immunization, the spleen was isolated from each mouse, homogenized, a cell suspension prepared, split into three wells (triplicate per mouse) and cultured in 96-well microtiter plates in the presence of 20 μg/ml of RSV pre-fusion protein for 5 days. The frequencies of RSV-specific Th1 (IFN-γ+CD4+ and TNF-α+CD4+) (A-B), Th2 cells (IL-4+CD4+, IL-5+CD4+, IL-10+CD4+) (C-E), Tfh (IL-21+ CD4+) (F) and Th17 (IL-17A+ CD4+) (G) cells were determined by flow cytometry after intracellular staining with the corresponding anti-cytokine. Error bars represent SD from n  =  5 mice. *P<0.5, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data were analyzed using Student’s t-test.