Figure 2. Topical inducible deletion of Skiv2l in epidermis results in localized inflammation in the skin.

(A) A schematic diagram showing experimental design for Skiv2l deletion in mouse skin by topical application of 4-OHT at 4 weeks old for 5 consecutive days. DMSO was applied on the contralateral skin as vehicle control. (B) H&E staining of 4-OHT- or DMSO-applied skin of Skiv2l^fl/flUBC-Cre/ERT2 mice and Skiv2l^fl/fl littermates 3 weeks after treatment. (C) Long section including both 4-OHT–treated and adjacent untreated skin of Skiv2l^fl/flUBC-Cre/ERT2 mice. Scale bar: 200 μm. (D) Skin-draining inguinal lymph node of 4-OHT– or DMSO-treated skin of Skiv2l^fl/flUBC-Cre/ERT2 mice and Skiv2l^fl/fl littermate controls.