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. 2022 Jan 18;132(2):e146071. doi: 10.1172/JCI146071

Figure 4. SAT cells and ABCs progressively and correlatively expand within TLTs following kidney injury.

Figure 4

(A and B) Representative FACS plots and quantification of the frequencies of (A) SAT cells and (B) ABCs and germinal center B cells (GCB cells) in aged kidneys at various time points following ischemic reperfusion injury (IRI). (C) The correlation between the frequencies of SAT cells and the sum of the frequencies of ABCs and GCB cells in A and B (n = 3/time point; day 0, 4, 24, 45, and 60). (D) Immunofluorescence (IF) of GFP and CD45, CD3ε, B220, CD11c, p75 neurotrophin receptor (p75NTR), CD21, Ki67, and p21 in the kidneys of aged Spp1-EGFP-KI mice subjected to IRI. The white arrows indicate the localization of TLTs. Magnified views of the outlined box are shown on the right. (E) IF of GFP and CD45; GFP, B220, and CD21; and quantification of the number of CD45+GFP+ cells and CD21– B cells within TLTs in the renal cortex in serial sections of aged Spp1-EGFP-KI mouse kidneys at various time points following IRI (n = 3/time point; day 0, 4, 24, and 60). (F) The correlation between the number of CD45+GFP+ cells and CD21– B cells in E. Scale bars: 50 μm (D and E). Data are presented as mean ± SD. Statistical significance was determined by trend test, and correlation was determined by Pearson’s correlation analysis. *P < 0.05; **P < 0.01.