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. 2022 Jan 18;132(2):e149711. doi: 10.1172/JCI149711

Figure 6. GO analysis of nonmyocyte single-cell RNA-Seq at 7 days following cardiac injury.

Figure 6

(A) GO analysis demonstrating principal pathways upregulated in CFs of control mice versus ENPP1CKO animals. (B) Dot plot demonstrating principal ECM genes upregulated in control versus ENPP1CKO CFs 7 days after cardiac injury. (C) Dot plot demonstrating differential expression of pro- and antiapoptotic genes in CFs at 7 days after injury. (D) Dot plot demonstrating differential expression of inflammatory genes in macrophages of control and ENPP1CKO hearts at 7 days after injury. (E) Masson trichrome staining showing expression of collagen (blue) in the apical region of hearts of mice at 7 days following injury and (F) quantitation of fibrotic scar (arrowheads) size at 7 days following injury (n = 5). Scale bar: 1 mm. (G) Immunofluorescent staining demonstrating CD68 (macrophages, arrowheads) and CD31 (endothelial, arrowheads) staining in control and ENPP1CKO animals at 7 days following injury and (H) quantitation of cells by histology (n = 5). Scale bars: 10 μm. Data are represented as mean ± SEM. **P < 0.01; *P < 0.05, 2-tailed Student’s t test (F and H).