TABLE 1.
Comparison of FRET-based PCR with conventional RFLP analysis
| Origin of isolate | Methoda | Genotype
|
|
|---|---|---|---|
| Wild-type VZV | Oka vaccine strain VZV | ||
| United States | FRET | 25 | 1 |
| RFLP | 25 | 1 | |
| Japan | FRET | 25 | 0 |
| RFLP | 25 | 0 | |
| Australia | FRET | 9 | 0 |
| RFLP | 9 | 0 | |
| Chad | FRET | 5 | 0 |
| RFLP | 5 | 0 | |
| Nepal | FRET | 5 | 0 |
| RFLP | 5 | 0 | |
| Congo | FRET | 5 | 0 |
| RFLP | 5 | 0 | |
| China | FRET | 3 | 0 |
| RFLP | 3 | 0 | |
| France | FRET | 1 | 0 |
| RFLP | 1 | 0 | |
a
FRET, FRET-based PCR with the ORF 62-based probes and primers described in Fig. 1; RFLP, SmaI digestion of the ORF 62 amplicon by RFLP analysis, as described in Materials and Methods.