Figure 6.

CoQ₀ attenuates LPS/ATP-stimulated ROS generation in RAW264.7 macrophages. (a) Cells were pretreated with CoQ₀ (10 μM) for 1 h and then stimulated with LPS (1 μg/mL) for 5 h followed by ATP (5 mM) for 1 h. The level of intracellular ROS was measured by DCF fluorescence using fluorescence microscopy (200x magnification). (b) Data are presented as fold change, and the results were calculated as the mean ± SD of three experiments, where ^∗∗∗p < 0.001, compared with untreated control cells, and ^###p < 0.001 compared with LPS/ATP-stimulated cells.