Figure 4.

Knockdown of AL033381.2 inhibited tumor growth in vivo. (a) Typical images of xenograft tumors formed by Huh-7 cells transfected with shCtrl or shAL033381.2 from each group of mice. (b, c) Tumor volume and tumor weight in each group of mice. (d) Typical H&E staining and IHC staining images of Ki-67, PCNA, Caspase 3, E-cadherin, N-cadherin, MMP2, and MMP9 in xenograft tumor tissues from each group of mice. ^∗∗P < 0.01. (e) The expression of E-cadherin, N-cadherin, MMP2, and MMP9 at the protein level in transfected cells with shCtrl or shAL033381.2 were examined by Western blot analysis.