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. 2021 Dec 9;132(1):140–153. doi: 10.1152/japplphysiol.00533.2021

Figure 5.

Figure 5.

A: phosphorylated (p)AktThr308/Akt in paired epitrochlearis muscles incubated ± 100 µU/mL insulin at 3 h postexercise (3hPEX). HFD, high-fat diet; LFD, low-fat diet; SED, sedentary. With insulin: *LFD-3hPEX > LFD-SED (P < 0.05) and HFD-3hPEX (P < 0.001). B: delta-insulin pAktThr308/Akt. *LFD-3hPEX > LFD-SED (P < 0.05); †LFD-3hPEX > HFD-3hPEX (P < 0.001). C: pAktSer473/Akt in paired epitrochlearis muscles incubated ± 100 µU/mL insulin at 3hPEX. With insulin: †LFD-3hPEX > HFD-3hPEX (P < 0.05). D: delta-insulin pAktSer473/Akt. †LFD-3hPEX > HFD-3hPEX (P < 0.05). Data were analyzed by 2-way analysis of variance within each insulin level (without or with insulin) or for delta-insulin values. Delta-insulin values = value with insulin − value without insulin from paired muscles. Tukey post hoc analysis was performed to identify significant differences. Values are means ± SD; n = 10 rats per treatment group. The representative blots are aligned with the group identification in the bar graph.