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. 2021 Dec 9;132(1):140–153. doi: 10.1152/japplphysiol.00533.2021

Figure 6.

Figure 6.

A: phosphorylated (p) Akt substrate of 160 kDa (AS160)Ser704/AS160 in paired epitrochlearis muscles incubated ± 100 µU/mL insulin at 3 h postexercise (3hPEX). HFD, high-fat diet; LFD, low-fat diet; SED, sedentary. *3hPEX vs. SED within each diet with no insulin (P < 0.01 for LFD group; P < 0.05 for HFD group); †3hPEX vs. SED within each diet with insulin (P < 0.05 for LFD group; P < 0.01 for HFD group). B: delta-insulin pAS160Ser704/AS160. C: pAS160Ser588/AS160 in paired epitrochlearis muscles incubated ± 100 µU/mL insulin at 3hPEX. *3hPEX vs. SED within each diet with no insulin (P < 0.01 for LFD group; P < 0.05 for HFD group); †3hPEX vs. SED within LFD group with insulin (P < 0.05). D: delta-insulin pAS160Ser588/AS160. E: pAS160Thr642/AS160 in paired epitrochlearis muscles incubated ± 100 µU/mL insulin at 3hPEX. †3hPEX vs. SED within each diet with insulin (P < 0.05). F: delta-insulin pAS160Thr642/AS160. Data were analyzed by 2-way analysis of variance within each insulin level (without or with insulin) or for delta-insulin values. Delta-insulin values= value with insulin − value without insulin from paired muscles. Tukey post hoc analysis was performed to identify significant differences. Values are means ± SD; n = 10 rats per treatment group. The representative blots are aligned with the group identification in the bar graph.