Fig. 7.
Results of Surface Plasmon Resonance experiments. (A-C), The TRMT2A RRM was coupled to a CM5 chip reaching 3000 RU. A concentration series from 0.5 to 8 μM spermine (A)/compound 12 (C) was injected, resulting in the illustrated, double-referenced binding curves. A representative curve from a triplicate measurement is shown. (B-D),The response at the equilibrium of the binding curve is plotted against spermine (B)/compound 12 (C) concentration. (E) A steady-state affinity model was fitted and allowed for the calculation of KD values.