Fig. 3.

Most of the MavN orthologs complemented the L. pneumophila mavN deletion mutant for intracellular growth. Intracellular competition assay in A. castellanii between L. pneumophila wild type strain JR32 containing a vector and the mavN deletion mutant complemented with: the L. pneumophila MavN (A); the L. longbeachae MavN ortholog Llo0254 (B); the L. micdadeii MavN ortholog Lmic0619 (C); the L. oakridgensis MavN ortholog Loak1149 (D) and the L. geestiana MavN ortholog Lgee2135 (E). All MavN orthologs were introduced on a plasmid under Ptac control in the same way as the L. pneumophila mavN gene. CFU – colony forming units. The data shown are representative of three independent experiments.