Fig. 5.

Intracellular growth complementation of the L. pneumophila legA3 deletion mutant by different LegA3 orthologs. Intracellular competition assay in A. castellanii between L. pneumophila wild type strain JR32 containing a vector and the legA3 deletion mutant complemented with: the L. pneumophila LegA3 (A); the L. longbeachae LegA3 ortholog Llo0584 (B); the L. micdadeii LegA3 ortholog Lmic2509 (C); the L. oakridgensis LegA3 ortholog Loak0513 (D) and the L. geestiana LegA3 ortholog Lgee0773 (E). All LegA3 orthologs were introduced on a plasmid under Ptac control in the same way like the L. pneumophila legA3 gene. CFU – colony forming units. The data shown are representative of three independent experiments.