Fig. 7.

Both LegA3 and LegA3C are required for maximal intracellular growth of L. pneumophila. Intracellular competition assay in A. castellanii between L. pneumophila wild type strain JR32 (or the wild-type strain containing a vector in the complementation experiments) and the legA3C-legA3 double deletion mutant (A); the legA3C-legA3 double deletion mutant containing the legA3C-legA3 genes (B); the legA3C-legA3 double deletion mutant containing the legA3 gene by itself (C); and the legA3C-legA3 double deletion mutant containing the legA3C gene by itself (D). The legA3C-legA3 operon, the legA3C and legA3 genes were all introduced on a plasmid under Ptac control. CFU – colony forming units. The data shown are representative of three independent experiments.