Skip to main content
. 2021 May 25;26(11):6365–6379. doi: 10.1038/s41380-021-01102-4

Fig. 2. Cell proliferation, DCX expression, and neuronal survival in the dentate gyrus of C57BL/6 mice upon different diet paradigms.

Fig. 2

Photomicrographs of BrdU+ cells in the dentate gyrus 24 h after the last BrdU injection in (A: a, c, e, g) or 4 weeks after the last BrdU injection (A: b, d, f, h) in mice fed ad libitum (AL) (A: a, b), under calorie restriction (CR) conditions (A: c, d), or under intermittent fasting (IF) conditions (A: e, f). g and h are higher magnification photomicrographs of the boxed area in e and f, respectively. The number of BrdU+ cells per DG in CR and IF conditions was superior to AL 24 h (B) and 4 weeks (C) after BrdU injections. IF was also superior to CR regarding number of BrdU+cells per DG 24 h post injection (B). Photomicrographs of DCX+ cells in the dentate gyrus of AL, CR, and IF mice (D). The number of DCX+ neuroblasts was also increased in the IF group in comparison with both AL and CR, respectively (E). Analysis of co-labeled cells with BrdU and the nuclear neuronal marker NeuN revealed that IF mice significantly increased the proportion of co-labeled cells when compared to AL and CR animals, respectively (F). Scale bars, 100 μm (a–f), 35 μm (g, h), 250 μm (D). BrdU bromodeoxyuridine, DCX doublecortin, DG dentate gyrus, NeuN neuronal nuclei protein. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.