Fig. 1. Specific knock-down of microglial Bmal1 (microgliaBmal1-KD) alters the expression of clock genes in adult male mice.
A Strategy used to isolate microglia in this study. ZT0 = lights on; ZT12 = lights off. B Relative expression of clock genes in microglia isolated from brains of wild-type C57BL/6J male mice. Shown are data obtained from microglia isolated every 3 h from ZT0 through ZT21 (n = 8 mice per group); data shown for points ZT0 and ZT24 are from the same samples. C Statistical analysis of rhythmic expression and acrophase determined for each of the clock genes; P < 0.05 was considered as representing significant rhythmicity. D Experimental strategy used for postnatal deletion of Bmal1 specifically in microglia and the time course for microglial isolation. E Representative images and quantification of western blots documenting immunoreactive Bmal1 and Histone H3 in isolated and CD11b-affinity purified microglia (n = 4 mice per group). F Expression of clock genes in microglia isolated from the brain of Ctrl and microgliaBmal1-KD mice at 3 weeks after the tamoxifen injections (n = 5–7 mice per group). Data are presented as means ± s.e.m. Green-colored * indicates a genotype effect; **P < 0.01, ***P < 0.001.