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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Trends Biochem Sci. 2021 Jul 16;47(2):124–135. doi: 10.1016/j.tibs.2021.06.008

Figure 4: From a single cryoEM dataset, 3-dimensional classification of projections revealed two distinct conformers representing two distinct GPCR-G protein interaction states representing two thermodynamically comparable conformers.

Figure 4:

In the canonical state (left, PDB ID 6OS9), the receptor engages the G protein in a prototypical fashion in which the nucleotide binding pocket is primed for GTP binding. In the non-canonical state (right, PDB ID 6OSA), the G protein heterotrimer is rotated by 45° compared to the canonical state, representing an intermediate ligand-bound receptor state along the G protein coupling pathway. TM: transmembrane helix; α-N: N-terminal alpha helix of G protein.