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. 2022 Jan 14;12:770. doi: 10.1038/s41598-022-04843-6

Figure 1.

Figure 1

Induction and proliferation of calli from mature zygotic embryo culture on 1/2 LV medium with 1 mg/L 2,4-D and 0.5 mg/L BA. (a) Culture of mature zygotic embryos of P. strobus. (b). Induction of calli on the surfaces of zygotic embryos after two weeks of culture. (c) Callus induction after subculture on new 1/2 LV medium with 1 mg/L 2,4-D and 0.5 mg/L BA. (d) Actively proliferated calli on 1/2 LV medium with 1 mg/L 2,4-D and 0.5 mg/L BA after 4 weeks of culture. Bar in (a) = 4 mm. Bar in (b) = 2 mm. Bar in (c) = 2 mm. Bar in (a) = 15 mm.