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. 2022 Jan 14;12:770. doi: 10.1038/s41598-022-04843-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Photos of callus its ground powders after drying. (a) Faded yellow callus after one month of culture on 1/2 LV medium with 1.0 mg/L 2,4-D with 0.5 mg/L BA. (b) Brown callus after 8 weeks of culture on 1/2 LV medium with 1.0 mg/L 2,4-D with 0.5 mg/L BA. (c) Dark brown callus after 12 weeks of culture on 1/2 LV medium with 1.0 mg/L 2,4-D with 0.5 mg/L BA. Photos on the right sides (a–c) are ground powders after drying. Bars in (a–c) = 20 mm.