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. 2022 Jan 14;12:770. doi: 10.1038/s41598-022-04843-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Light microscopic observation of yellow and dark brown calli. (a) Faded yellow callus after one month of culture constituted with cell mass with spherical cell structure with dense cytoplasm. (b) Callus constituted with cell mass with spherical cell structure with brown pigment. Right side of photos are enlarged views. Bars in (a,b) = 50 μm.