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. 2022 Jan 14;12:770. doi: 10.1038/s41598-022-04843-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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GC/MS analysis of the production of DPME and PME in P. strobus calli by prolonged culture. (a) GC chromatogram of extract from yellowish calli after 4 weeks of culture. (b) GC chromatogram of callus extract from pale brown callus after 8 weeks of culture. (c) GC chromatogram of callus extract from dark brown calli after 12 weeks of culture. (d) GC chromatogram of authentic standards of DPME and PME. (e) Mass fraction of a DPME peak in callus and DPME standard. (f) Mass fraction of a PME peak in callus and PME standard.