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. 2022 Jan 14;12:770. doi: 10.1038/s41598-022-04843-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Growth of callus, accumulation of pinosylvin strilbene and expression of genes involved in pinosylvin strilbene biosynthesis in the different concentrations of MeJA. (a) Growth of calli grown in different concentrations of MeJA after 4 weeks of culture. (b,c) qPCR analysis of PsSTS and PsPMT genes in P. strobus callus. The qPCR data were normalized to β-actin gene expression. The vertical bars indicate the SE based on three biological replicates. (d) DPME accumulation in cell suspension during different days of culture in medium with or without 100 µM MeJA.