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. 2022 Jan 14;12:762. doi: 10.1038/s41598-021-03934-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of LK-DFBA metabolite concentration predictions against ODE data and L. lactis experimental data when fitted to noiseless and noisy ODE data. Panels A, B, and C depict concentration profiles for LK-DFBA (HP) (solid red line) and the ODE model (dashed black line) compared to experimental data (blue circles) for initial glucose concentrations of 20 mM, 40 mM, and 80 mM, respectively, when LK-DFBA is fitted to noiseless data. Panels D, E, and F depict concentration profiles for LK-DFBA (NLR) on 10 noisy datasets (nT = 15, CoV = 0.15) and the ODE model compared to experimental data. The mean concentration profile (solid green line) is shown with each of the concentration profiles (solid red lines) from the 10 noisy datasets.