Fig. 5. Nuclear translocation of Rac1 was induced by radiation, which subsequently prolonged the residence time of p53 in the nucleus.

MLE-12 cells were pretreated with PBS or NSC23766 (NSC, 100 μM) for 2 h and then went through 10 Gy of radiation. A At 0–2 h after radiation, nucleoprotein, and cytoplasmic protein were separated for detection of the subcellular distribution of Rac1 and p53. B At 2 h after radiation, the translocation of Rac1 and p53 were detected with or without NSC23766 (NSC, 100 μM) pre-treatment. C Cells were collected from 0 to 6 h after radiation for immunofluorescence analysis of the translocation of Rac1 and p53. Green and Red fluorescence represented Rac1 and p53, respectively.