TABLE 1.

Bacterial strains and oligonucleotides used in this study

Strain or oligonucleotide	Descriptiona	Reference or source
C. rodentium (previously described)
DBS100	Prototype TMCH isolate, ATCC 51459, original biotype 4280	1
DBS125	University of Missouri–Columbia isolate 1136-89, biotype 4280	33
DBS126	University of Missouri–Columbia isolate 2896-81, biotype 4280	33
CDC1843-73T	ATCC 51116 (species 9 type strain)	5
CDC2643-76	ATCC 51637	5
CDC3311-75	ATCC 51638	5
C. rodentium (this study)
DBS425	University of Washington 95-4234, from blood	20
DBS426	University of Washington 95-4240, from liver	20
DBS427	University of Washington 95-4241, from blood	20
DBS428	University of Washington 95-4243, from liver	20
DBS494	University of Washington 96-4212B	20
DBS495	University of Washington 96-4212W	20
DBS496	University of Washington FVO 10, from colon	20
DBS500	University of Washington, 6-18-96	20
DBS557	Oak Ridge National Laboratory 3431e, from colon	This report
MPEC	E. coli O115a,c:K(B) strain Ex-33	15
Citrobacter type strains
CDC621-64	C. freundii, ATCC 8090 (species 1)	5
CDC3613-63	C. koseri, ATCC 27156 (species 2)	5
CDC9020-77	C. amalonaticus, ATCC 25407 (species 3)	5
CDC2991-81	C. farmeri, ATCC 51112 (species 4)	5
CDC460-61	C. youngae, ATCC 29935 (species 5)	5
CDC80-58	C. braakii, ATCC 51113 (species 6)	5
CDC876-58	C. werkmanii, ATCC 51114 (species 7)	5
CDC4696-86	C. sedlakii, ATCC 51115 (species 8)	5
CDC4693-86	C. gillenii, ATCC 51117 (species 10)	5
CDC2970-59	C. murliniae, ATCC 51118 (species 11)	5
E. coli DH5α	F− φ80dlacZΔ endA recA hsdR supE thi gyrA Δ(lacZYA argF)	BRL
Oligonucleotides
CF2974	5′-CGGGATCCAATAAGACTGAAGCAAC-3′	This study
SAL013	5′-GCTAAGCTTATATGATTAGAC-3′	This study
ESPB1	5′-TGAGACAGTTGGCACATTGC-3′	28
ESPB2	5′-TGGTGGTACAACTCTTCGAGC-3′	28
BOXA1R	5′-CTACGGCAAGGCGACGCTGACG-3′	34
ERIC1R	5′-ATGTAAGCTCCTGGGGATTCAC-3′	34
ERIC2	5′-AAGTAAGTGACTGGGGTGAGCG-3′	34
REP1R-I	5′-IIIICGICGICATCIGGC-3′	34
REP2-I	5′-ICGICTTATCIGGCCTAC-3′	34

^aI, inosine.