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. 2022 Jan 14;12:721. doi: 10.1038/s41598-021-04627-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Specified bacteria were incubated for 30 min with fluorescein-tagged GP12 (25 μM) and analyzed using flow cytometry. (B) Various strains of P. aeruginosa were incubated for 30 min with fluorescein-tagged GP12 and analyzed using flow cytometry. Please note that the levels for PAO1 were the same results measured in the screen of bacteria in panel (A). (C) P. aeruginosa (PAO1) was incubated with GP12, washed with PBS, and fluorescence was monitored over 6 h. Inset, fluorescence microscopy image of P. aeruginosa (PAO1) treated with fluorescein-tagged GP12. Data are represented as mean ± SD (n = 3). p-values were determined by a two-tailed t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns not significant). (D) Gram-positive bacteria and P. aeruginosa (PAO1) were incubated for 30 min with fluorescein-tagged GP12 and analyzed using flow cytometry.