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. 2022 Jan 14;12:721. doi: 10.1038/s41598-021-04627-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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E. coli with various genetic deletions in the biosynthetic pathway of LPS were tested for their ability to bind fluorescently labeled GP12. Top, specified mutants were incubated for 30 min with fluorescein-tagged GP12 and analyzed using flow cytometry. Data are represented as mean ± SD (n = 3). Bottom, schematic representation of LPS that relates to the mutant shown in the top panel. The red spheres represent phosphorylation sites.