Figure 4.

(A) Schematic representation of the lipid A and core oligosaccharide region of E. coli and P. aeruginosa. (B) QCM-D frequency shift (− ∆F) upon GP12 adsorption to gold coated sensor (red) and BSA adsorption on gold (purple). BSA injected after adsorption of GP12 minimally adsorbed to the surface (blue), indicating near complete GP12 surface coverage. (C) QCM-D frequency shift (− ∆F) as a function of time for liposomes binding GP12. Liposomes containing 20 wt.% P. aeruginosa LPS (green) or 20 wt.% E. coli LPS (blue) bind the GP12-coated surface, while LPS-free liposomes (red) do not bind. (D) QCM-D frequency shift (− ∆F) as a function of time for non-liposomal hydrated LPS and POPC:POPG:POPE liposomes binding to GP12. Suspensions of 0.25 mg mL⁻¹ P. aeruginosa LPS (green) or E. coli LPS (blue) bind to the GP12-coated surface, while LPS-free liposomes (red) do not bind. All QCM-D binding studies were carried out in triplicate and representative binding curves are shown.