Figure 5.

Binding of P. aeruginosa to agarose beads was assessed by measuring the fluorescence levels (A) and by visual inspection (B). P. aeruginosa was incubated for 60 min at 37 °C in the presence of agarose beads modified with GP12 or control beads in PBS. After this incubation period, the reagent resazurin was added and fluorescence was measured after an incubation time of 30 min. Fluorescence was measured by 550 nm excitation and 605 nm emission. Data are represented as mean ± SD (n = 3).