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. 2021 Dec 17;298(1):101499. doi: 10.1016/j.jbc.2021.101499

Figure 3.

Figure 3

Overexpression of ALKBH1 promotes adipogenesis.A, Western blot confirms the successful overexpression of ALKBH1 in hMSCs. B, quantitative analysis of oil red O staining in hMSCs after 14 days of adipogenic differentiation (n = 3). Scale bar: 25 μm. C, qRT-PCR analysis of adipogenic related genes CEBPA, PPARG, PLIN1, ADIPOQ in ALKBH1-overexpressed hMSCs (n = 3). D, Western blot shows overexpression of ALKBH1 in 3T3-L1 cells. E, representative images and the OD values of oil red O staining in 3T3-L1 cells (n = 3). Scale bar: 25 μm. F, qRT-PCR analysis of Cebpα, Pparγ, Plin1, Adipoq in ALKBH1-overexpressed 3T3-L1 cells (n = 3). G, macroscopic identification and quantitative analysis of ectopic fats after 6 weeks of sternal subcutaneous injection. Nude mice were bilateral transplanted with Vector and Lv-ALKBH1 3T3-L1 cells (n = 6). Scale bar: 5 mm. H, hematoxylin and eosin staining (top) and GFP staining (bottom) of fat pads isolated from nude mice. Scale bar: 25 μm. The p values were calculated by two-tailed Student's t test. Scatter plots show individual data points ± SD.