Figure 1.

The PcG protein CBX2 is significantly upregulated in LUAD, and its depletion suppresses the growth and invasion of LUAD cells

(A) Heatmap showing the expression of 7 differentially expressed (FDR <0.01, fold change ≥2 and CPM >30) histone methylation-related genes in LUAD tumor samples and normal samples. (B) The differences of CBX2 and EZH2 mRNA expression between the paired tumor (n = 58) and normal (n = 58) samples (pairs are connected by gray dash lines). The p values were estimated using the R package NOISeq. ∗∗∗p ≤ 0.001. (C) The differences of CBX2 and EZH2 mRNA expression between normal (n = 58, tumor adjacent), DMF (n = 353), and DM (n = 25) samples. (D-G) A549 cells transfected with control, CBX2, or EZH2 siRNAs were applied. (D) CCK-8 assays were used to examine the cell viability of the transfected A549 cells. (E) EdU incorporation assays were used to examine the proliferation of the transfected A549 cells. (F) FITC-labeled annexin V and propidium iodide were used to stain the transfected A549 cells, and flow cytometry was used to detect apoptosis. (G) The cell invasion was examined by counting the transmigrated cells under a microscope. In (D-G), each bar represents the mean ±SD of three independent biological replicates. ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001 (Student's t test). (H) Protein expression of EMT markers was determined by western blotting in A549 cells transfected with control, CBX2, or EZH2 siRNAs. The molecular weights are indicated on the left side of the plot.