Figure 2.

CBX2 knockdown significantly suppresses LUAD growth and metastasis in vivo

(A) Western blot analysis validating the inhibitory effect of CBX2 sgRNAs on the protein expression of CBX2 in A549 cells. (B) Control A549 cells or A549 cells expressing CBX2 sgRNAs (A549-sgCBX2) were transplanted into female athymic nude mice (BALB/c, Charles River; ages between five and six weeks; six mice per group). (C) Tumors were measured at the indicated times using a vernier caliper. According to the formula, V = π/6 × length × width², the volume of tumor was calculated. Error bars represent the mean ± SD of six animal measurements. ∗p ≤ 0.05; ∗∗p ≤ 0.01 (Student's t test). (D) The tumors dissected from the mice were weighed. Each bar represents the mean ± SD of six animal measurements. ∗∗∗p ≤ 0.001 (Student's t test). (E) Representative in vivo bioluminescent images showing the difference in metastasis between the A549-Luc and A549-sgCBX2-Luc mice group. After six weeks of initial implantation, the metastasis of tumor cells was quantified using bioluminescence imaging. Each bar represents the mean ± SD of three animal measurements. ∗p ≤ 0.05 (Student's t test).