Fig. 2.

Overview of the arrayed 5440 compounds library screen A) Schematic representation of the screening procedure, at 1.67 ​μM per compound. B) Ranking of compounds by number of plaques and total GFP intensity. The cut-off value was set at a Z score of −3 (see Methods). MB, MPA and POS are highlighted in blue. Data points represent means of 4 independent biological replicates. C) Hit classification. Most of the hits classified as anticancer agents or modulators of metabolism. D) Example images of cells treated with the indicated compounds as described in A. In the negative control (DMSO), clusters of GFP positive cells represent infection foci (green). Nuclei are represented in blue. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)