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. 2022 Jan 15;22:27. doi: 10.1186/s12935-022-02455-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — hsa_circ_0000231 promotes cell proliferation and tumor growth in vitro and in vivo in CRC. A and B CCK8 assay was performed to detect the effect of hsa_circ_0000231 on cell proliferation. The number of cell proliferation decreased significantly in SW480 cells or SW620 cells in the si_circ_0000231 and si_circ_0000231 combined with si-mRNA (NM_018287) groups (si-both) compared with the control group. C and D Colony formation assays were executed to detect the proliferation of cells transfected with indicated vectors. The result showed that the downregulation of hsa_circ_0000231 could markedly reduce the cell cloning capabilities of SW620 and SW480 compared with the negative control group (si-NC). E and F Apoptosis rate was analyzed by flow cytometry after downregulation of hsa_circ_0000231. The results showed that knocking down hsa_circ_0000231, there was no significant difference in apoptosis of the two groups of cells. G and H The cell cycle progression was analyzed by flow cytometry after downregulation of hsa_circ_0000231. The cell cycle detection by flow cytometry revealed that the percentage of G1 cells obviously increased after inhibiting the expression of hsa_circ_0000231 in SW480 and SW620 cells. I Images of xenograft tumors of each group (n = 5). J The difference in tumor volume in different intervention groups. The result showed that the tumor volume in the hsa_circ_0000231 group was significantly higher than that in the mock group. K The difference in tumor weight in different intervention groups. The result showed that the tumor weight in the hsa_circ_0000231 group was significantly higher than that in the mock group. Data were showed as mean ± SD, *p < 0.05, **p < 0.001