Fig. 5.

IGF2BP3 could bind to hsa_circ_0000231 and CCND2, as an RBP to protect the stability of RNA. A and B Anti-IGF2BP3 RIP was executed in SW620 and SW480 cells followed by western blot and qRT-PCR to detect IGF2BP3 protein, hsa_circ_0000231 and CCND2, respectively. C and D RNA pull-down was executed in SW620 and SW480 cells, followed by qRT-PCR to detect the enrichment of hsa_circ_0000231 and CCND2, respectively. E and F qRT-PCR was used to detect expression of hsa_circ_0000231 and CCND2 after overexpression of IGF2BP3. G–J Western Blot assay was conducted to confirm the relative expression of CCND2 transfected with indicated vectors, miRNAs or inhibitors in SW480 cells. Data were showed as mean ± SD, *p < 0.05, **p < 0.001