Fig. 2.

SHC4 promotes proliferation, anchorage-dependent colony formation, and regulates cell cycle genes in HCCs. A, B The cell viability of indicated stable cells was measured by CCK-8 assay at different time points. ***P  < 0.001, One-way ANOVA or Student t test. C, D The anchorage-depend colony formation assay was performed to assess the effects of SHC4 knockdown or overexpression on clone formation ability of HCC cells. Representative images of colonies were shown (upper panel) and the number of colonies were counted (lower panel). **P  < 0.01, ***P  <  0.001, One-way ANOVA or Student t test. E, F Effect of SHC4 knockdown or overexpression on cell cycle genes in indicated cells was identified by western blotting. Cell lysates were subjected to western blotting for c-MYC, cyclin D1, and p21