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. Author manuscript; available in PMC: 2022 Jan 15.
Published in final edited form as: Circ Res. 2014 Jun 5;115(4):450–459. doi: 10.1161/CIRCRESAHA.115.304262

Figure 4. The MTS of Arg2 is located on N-terminus and includes amino acids 1-22 or 1-40.

Figure 4

HAEC were transfected with Full length Arg2, or either the 1-22 or the 1-40 N terminal deletion mutants, and analysis of A) western blotting, B) subcellular fractionation of full length and 1-22 truncated Arg2, and C) immunofluorescence using anti-FLAG antibody (green) and mitotracker red are shown. Full length Arg2 was localized almost exclusively to mitochondria and both truncated mutants were predominantly cytosolic (DAPI-labeled nuclei (blue) are shown in the merged images). Scale bar denotes ~40 microns.