Figure 5.
TP53 R273C -mutant tumors show differential gene expression relative to other TP53 mutations, with sex-specific differences. LIMMA analysis of RNA expression data from the TCGA LGG cohort was used to compare gene expression in TP53R273C astrocytomas to those lacking a codon 273 mutation. (A–B) Analysis of female tumors (left column) and male tumors (right column) demonstrates a cloud of down-regulated genes, more prominent in females, which gene set enrichment analysis showed to be composed of proliferation-related pathways. (C) Direct p53 target genes and the Hallmark p53 pathway gene set showed modest effects, mainly in females, including significant upregulation of p21. (D) The largest up-regulated Hallmark gene set is TNF-α signaling via NF-κB.