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Primers used for H19, PG3, and SNRPN methylation analysis
| Imprinted gene | Primer sequence | Amplicon length (base pair) | Number of CpGs site |
|---|---|---|---|
| H19 | Forward | 253 | 6 |
| AGGGTTTTTGGTAGGTATAGAG | |||
| Reverse | |||
| CCTATTCCCAAATAACCCC | |||
| Sequencing | |||
| GTGGAATAGGAAGTGGT | |||
| PEG3 | Forward | 154 | 4 |
| GGTGTAGAAGTTTGGGTAGTT | |||
| Reverse | |||
| ACTCACCTCACCTCAATACTAC | |||
| Sequencing | |||
| GTTTATTTTGGGTTGGT | |||
| SNRPN | Forward | 220 | 7 |
| GGGAGGGAGTTGGGATTTTTGTA | |||
| Reverse | |||
| AAACCACCCACACAACTAACCTTAC | |||
| Sequencing | |||
| GGAGTTGGGATTTTTGTAT |
