FIGURE 3.

Vitamin B12 drives the increased osmotic tolerance and life span of flr‐4(n2259) grown on HT115, in a p38‐MAPK pathway‐dependent manner. (a) The flr‐4(−) worms are more osmo‐tolerant compared to wild‐type when grown on HT115. The flr‐4(−);sek‐1(km4) worms are not tolerant to osmotic stress, showing that the increased osmotic tolerance of flr‐4(−) is dependent on the p38‐MAPK pathway. Osmotic stress was given to the worms at L4 stage by transferring them to 350 mM NaCl for 10 min. The recovery of the worms on NGM plates is plotted as a function of time. (b) No difference in osmotic tolerance is observed in the strains when grown on OP50. (c) The flr‐4(−) worms grown on OP50 supplemented with B12 is more osmotic tolerant compared to non‐supplemented worms. This increased tolerance is also dependent on the p38‐MAPK pathway as no osmotic tolerance was observed in flr‐4(−);sek‐1(km4). (d) The flr‐4(−) worms have increased life span only when grown on HT115 or B12‐supplemented OP50. (e) The flr‐4(−) worms have increased life span when grown on OP50 supplemented with B12. The supplementation was not able to increase life span in the flr‐4(−);sek‐1(km4). Life span and osmotic tolerance assays were performed at 20°C. One of three biologically independent experiment shown. Life span summary is provided in Table S1. Summary of osmotic tolerance assay is provided in Table S2. Experiments were performed at 20°C. Source data is provided as a source data file