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. 2021 Dec 13;21(1):e13529. doi: 10.1111/acel.13529

FIGURE 6.

FIGURE 6

CiPVT1 delayed endothelial cell senescence by regulating the miR‐24‐3p/CDK4/pRb axis. (a and b) Western blot analysis of the levels of CDK4 and pRb in HUVECs and HCAECs transfected with si‐NC, si‐ciPVT1‐1, and si‐ciPVT1‐2. (c and d) Western blot analysis of the levels of CDK4 and pRb in HUVECs and HCAECs transfected with circControl (circCon) or ciPVT1‐GFP lentivirus. (e and f) MiR‐24‐3p could significantly suppress the expression of CDK4 and pRb, and the suppression was retarded after overexpression of ciPVT1 in HUVECs and HCAECs. (g) CDK4, pRb protein expression in blood vessels of young (n = 7) and old (n = 4) individuals. Data are presented as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001