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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Ultramicroscopy. 2020 Nov 15;221:113165. doi: 10.1016/j.ultramic.2020.113165

Figure 2: Scanning method and synchronization.

Figure 2:

(a) A 2D lateral scan is defined over a 10x10μm area with 10 scan lines. The scan definition is such that when the resulting data is formatted into a 2D image, each pixel value represents light collected from the center of that pixel. The scanning axis (X in this case) also extends before and after the scan area of interest to allow the triggering system which eliminates “warping” at the edges. Note that the range for each trigger extends slightly beyond the scan area, allowing a trigger “hand off’ between scan lines such that the trigger bounce does not signal the start of the next scan line collection. (b) Each piezo (sample and AFM) are programmed to internally record their positions and report the result at the end of the scan. To be sure the scan is not only spatially, but also temporally synced, the 2D scan is separated into each axis and plotted against time, X shown in (c) and Y shown in (d). The traces verify that the two piezo stages are synchronized both spatially and temporally.