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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Semin Cell Dev Biol. 2021 Sep 3;121:153–160. doi: 10.1016/j.semcdb.2021.08.013

Figure 2. MPRA can characterize variant effects on gene regulation in a high-throughput manner.

Figure 2.

MPRA libraries consist of a regulatory element that contains a genetic variant, promoter, reporter gene (GFP), and 10–20bp barcodes. These libraries are introduced to the cells of interest, from which RNA is extracted. Barcode expression is quantified to measure the variant effects on reporter gene expression.