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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Dev Biol. 2021 Dec 1;482:28–33. doi: 10.1016/j.ydbio.2021.11.007

Figure 2. Exogenous Vasa mRNA is not retained selectively anywhere in the embryo.

Figure 2.

A) Exogenous Vasa-mCherry protein is enriched in the PE and in some cells of the anterior pouches (AP), white arrow. B) Fluorescent in situ hybridization of larvae expressing Vasa-mch using the mCherry probe detects Vasa-mCherry mRNA in every cell of the larva. C) A probe for mcherry used as a negative control shows no endogenous mcherry gene activity in wild type larvae. D) Detection of endogenous vasa mRNA in wild type larvae using a vasa antisense probe showing that vasa transcripts are expressed in the PE and in some cells of the AP (white arrows). Asterisk indicates non-specific fluorescence in the stomach of fed larvae. Scale bars = 50 μm. Dotted white lines highlight the PE. All figures show larvae 90–96h old.